Scientific Area Sentinel Diagnostics

Evaluation of NGAL Test ™, a fully-automated neutrophil gelatinase-associated lipocalin NGAL immunoassay on Beckman Coulter AU 5822

Sentinel Diagnostics — 08/05/2012 11.36.00

Giuseppe Lippi, Rosalia Aloe, Antonietta Storelli, Gianfranco Cervellin and Tommaso Trenti

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Preliminary evaluation of a new immunoturbidimetric kit for the measurement of NGAL SIBioC 2011, Parma - Italy

Sentinel Diagnostics — 05/12/2011 16.01.00

C.Villa, G.Scarsi • Download attachment

A Rapid and Innovative Method for the Identification of the Commonest G6PD Italian Mutations SIBioC 2011, Parma - Italy

Sentinel Diagnostics — 24/11/2011 17.49.00

A. Minucci, L.Gentile, S. Rocchetti, C.Zuppi, B.Giardina, E. Capoluongo • Download attachment

A New Laboratory Friendly Platform to Detect the Celiac Disease associated HLA-DQ2 and DQ8 Haplotype SIBioC 2011, Parma - Italy

Sentinel Diagnostics — 16/11/2011 14.05.00

D.Bozzato, F.Navaglia, E.Rossi, M.Gramegna, M.Pelloso, R.favero, E.Greco, A.Padoan, S.Moz, P.Fogar, C-F.Zambon, D.Basso, M. Plebani • Download attachment

A New and Easy Way to Molecular Testing SIMeL 2010, Verona - Italy

Sentinel Diagnostics — 16/11/2011 12.22.00

M. Gramegna, A. Moiana • Download attachment

PCR-based Kit for the Screening and Typing of Malaria Infections EARCR 2009, Triuggio - Italy

Sentinel Diagnostics — 16/11/2011 12.20.00

M.Gramegna, A. Moiana, L. Coltella, C.Russo, S.Gatti • Download attachment

A New Universal and Self - Containing Lyophilized PCR Mix SIBIOC 2010, Rome - Italy

Sentinel Diagnostics — 16/11/2011 12.14.00

M. Gramegna, E. Castiglioni, A. Moiana, M. Zanussi, M. Ferrari • Download attachment

Validation of a New Freeze-Dried PCR Universal Master Mix AACC 2010, Anaheim - California

Sentinel Diagnostics — 16/11/2011 12.09.00

M. Gramegna, E. Castiglioni, A. Moiana, M. Zanussi, M. Ferrari
Sentinel Ch. has recently developed a new proprietary freeze-drying system for the manufacturing of ready-to-use PCR Universal Master Mixes. Usually the PCR mixes need to be stored at -20°C or + 2/8 °C. This means request for refrigerators and freezers in the labs, and controlled temperature in case of shipment. The new system is able to maintain the activity of the mix with no loss of performance at room temperature. The aim of the present study is to validate the performances of the freeze-dried mixes for the main molecular applications in research or diagnostic laboratories. • Download attachment

Innovative Real Time PCR Assay for the Screening and the Drug Reesistance Detection of Mycobacteria SIBioC 2011, Parma

Sentinel Diagnostics — 16/11/2011 12.05.00

A.Moiana, M.L.Incandela, S.Buroni, M.R.Pasca, M. Gramegna, G.Riccardi • Download attachment

Malaria Infection Diagnosis: Comparison between traditional methods and a new freeze-dried PCR Multiplex test IFCC 2011, Berlin - Germany

Sentinel Diagnostics — 16/11/2011 11.59.00

M.Gramegna, A. Moiana, F.A.Solari, S. Gatti • Download attachment

PCR-based kit for the early diagnosis of Toxoplasma infections EUROMEDLAB 2009, Innsbruck- Austria

Sentinel Diagnostics — 16/11/2011 11.37.00

M.Gramegna, A.Moiana, F.Genco, V.Meroni

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Evaluation of a New PCR-based Assay for the Screening and Typing of Malaria Infections AACC 2009, Phoenix - Arizona

Sentinel Diagnostics — 16/11/2011 11.29.00

M. Gramegna, A. Moiana, L. Coltella, C. Russo, S. Gatti • Download attachment

Not Invasive Doagnosis of Leishmania Infection by a novel PCR-based Assay AACC 2009, Phoenix - Arizona

Sentinel Diagnostics — 16/11/2011 11.28.00

M. Gramegna, A. Moiana, S. Gatti, L. Coltella, C. Russo • Download attachment

Evaluation of a new Syphilis assay on Vitros 5600 Integrated System

Sentinel Diagnostics — 23/06/2011 17.16.00

G. Longo, C. Bitella, M. La Motta, R. Lucini, F. Rota, A. Godani Serra, E. Vigo • Download attachment

Development of a PCR test for the diagnosis of Toxoplasma Gondii infections

Sentinel Diagnostics — 23/06/2011 16.43.00

A. Moiana, M. Gramegna, F. Genco, V. Meroni • Download attachment

Evaluation of diagnostic accuracy of screening by fecal occult blood testing FOBT

Sentinel Diagnostics — 17/05/2011 15.11.00

T. Rubeca, S. Rapi, M. Confortini, M. Brogioni, G. Grazzini, M. Zappa, D. Puliti, G. Castiglione, S. Ciatto
Evaluation of a new immunological quantitative fecal occult blood testing assay FOB Gold - Sentinel compared to the assay currently employed OC-Hemodia - Eiken for colorectal cancer screening. • Download attachment

Evaluation of Sentinel ACP-NP Assay on AU400 and AU2700 Systems

Sentinel Diagnostics — 05/10/2010

A. Cugini, L. De Angelis, F. Vespasiani, M. Valdambrini, E. Bianchi, G. Fumagalli
Evaluation of Total Acid Phosphatase (ACP) and Non Prostatic Acid Phosphatase (ACP-NP) assay from SENTINEL CH. on Beckman Coulter AU400 and AU2700 was performed versus quality specifications based on biological variability, according to Ricos. • Download attachment

Evaluation of Sentinel Cholinesterase Liquid Assay on LX20 and DXC600 Systems

Sentinel Diagnostics — 17/09/2010

F. Rota, E. Marelli, A. Cugini, F. Vespasiani, M. Valdambrini
Analytical performance of Sentinel Cholinesterase Liquid assays was evaluated on the Beckman Coulter Synchron® LX20 and UniCel® DXC600 Clinical Chemistry Systems. Goals of the study were to verify the analytical performance and to put on the market a validated CE marked application. • Download attachment

Evaluation of Sentinel CK-MB UDR Assay on LX20 and DXC600 Systems

Sentinel Diagnostics — 17/09/2010

F. Rota, E. Marelli, A. Cugini, F. Vespasiani, L. De Angelis
Analytical performance of Sentinel CK-MB UDR assay was evaluated on the Beckman Coulter Synchron® LX20
and on UniCel® DXC 600 Clinical Chemistry Systems. Goals of the study were to verify the analytical performance and establish agreement of this assay with the desirable analytical specification based on biological variability as given by Ricos. The test is proposed as liquid, bi-reagent, ready-to-use and doesn’t require calibration procedure. • Download attachment

New Syphilis Assay on Vitros 5600 Clinical Chemistry System

Sentinel Diagnostics — 22/10/2009

R. Lucini, F. Rota, C. Bitella, A. Godani Serra, E. Vigo
A new homogeneous immunoassay for detection of primary infection of "Treponema pallidum" evaluation on Vitros® 5600 integrated system.
Evaluation of the analytical performances and diagnostic accuracy in comparison to a Haemagglutination Assay TPHA.

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Programa Piloto de Preveciòn de Càncer de Colòn y Recto

Sentinel Diagnostics — 10/03/2009

J. Cruzado Quevedo, F. Pérez Riquelme, E. Torrella Cortès, M. C. Cavas Martìnez, P. Esteban Garcìa, J. J. Gutiérrez Garcìa, e Grupo Investigaciòn PCaColon y Recto
Experience and preliminary resuts of the pilot program for the prevention of Colorectal Cancer in the Salud VI area of the Spanish Region of Murcia. The Salud VI area covers 13 Zonas de Salud and 1 District Hospital for a total of 35.771 people. • Download attachment

Small Dense LDL-cholesterol: a superior diagnostic marker

Sentinel Diagnostics — 24/02/2009

Yasuki Ito, Yosuke Meguro, Kunio Kamata, Tsutomu Hirano, Makoto Kinoshita, Mariko Shiba, Tetstuo Shoji, Shinji Koba, Akira Tanaka, Gen Yoshino
Small dense LDL-cholesterol as a superior diagnostic marker for familial combined hyperlipidemia: sd LDL-C was mesured by s LDL-C'SEIKEN' using the heparin-magnesium precipitation method and mean LDL diameter was determined by the 2-16% gradient gel electrophoresis. Comparison of the sensitivity and specificity for discriminating familial combined hyperlipidemia (FCHL) from hyperlipidemia among four different parameters. • Download attachment

FOBT Common Guaiac vs. Immunochemical Test

Sentinel Diagnostics — 23/02/2009

H. J. Roth, H. Schmidt-Gayk
Fecal Occult Blood Test (FOBT) study: common guaiac versus immunochemical test. Higher sensitivity of the more recently developed iFOBTs simplifies and improves compliance in participation in the colon-rectal cancer (CRC) screening programs by thresholds adjustment versus sensitivity and specificity balance. • Download attachment

Comparison of CT colonography, colonoscopy, sigmoidoscopy and FOBT for adenoma detection

Sentinel Diagnostics — 11/02/2009

A. Graser, P. Stieber, D. Nagel, C. Shafer, D. Horst, C. R. Becker, K. Nikolaou, A. Lottes, S. Geisbusch, H. Kramer, A. C. Wagner, H. Diepolder, J. Schirra, H. J. Roth, D. Seidel, B. Goke, M. F. Reiser, F. T. Kolligs
Prospective trial for comparison of the performance characteristics of 5 different screening tests in parallel for the detection of advanced colonic neoplasia: CT colonography (CTC), colonoscopy (OT), flexible sigmoidoscopy (FS), faecal immunochemical stool testing (FIT) and faecal occult blood testing (FOBT). • Download attachment

Colorectal Cancer Screening By Quantitation Of Haemoglobin In Card

Sentinel Diagnostics — 09/01/2009

C. M. Matthew, K. McKay, R. J. C. Steele, C. G. Fraser
Screening for colonrectal cancer is increasing all over the world: guaiac faecal occult blood tests (gFOBT) are the most frequently used, while new faecal immunochemical tests (FIT) are now available for the quantitation of haemoglobin in faeces by collection in easy to use primary tubes. • Download attachment

Assessment of an Affinity Based Point of Care System for HbA1c

Sentinel Diagnostics — 16/09/2008

A. Mosca, R. Paleari, G. Passerini, F. Ceriotti
Evaluation of the analitical performance of a new Point of Care system (POCT) Afinion AS100 Analyzer (Axis-Shield PoC AS, Oslo, N), based on boronate affinity chromatography. The system takes 3 minutes per test, requires 1.5 μL whole blode per analysis, does not suffer interference in presence of HbS and HbC and reports National Glycohemoglobin Standardization Program (NGSP) aligned values. • Download attachment

Evaluation of SENTINEL CH. special assays on the ABBOTT c16000 Clinical Chemistry System

Sentinel Diagnostics — 09/09/2008

R. Lucini, R. Dioli, E. Marelli, F. Rota, M. Fritz, H. Troonen
Optimization of clinical laboratory workflow through consolidation of assays on a high-throughput analyzer as the recently introduced ABBOTT ARCHITECT c16000. • Download attachment

Preliminary Evaluation of a new Cystatin C Assay on the ABBOTT Clinical Chemistry Systems

Sentinel Diagnostics — 09/09/2008

R. Lucini, R. Dioli, L. De Angelis, F. Rota, M. Fritz, H. Troonen
A new Cystatin C assay evaluation on the ABBOTT ARCHITECT cSystems and AEROSET Clinical Chemistry Systems.Verification of the analytical performance and agreement with the NACB's guidelines for Emerging Biomarkers of Cardiovascular Disease and Stroke. • Download attachment

Evaluation of MULTIGENT Lithium assay on the ABBOTT Clinical Chemistry Systems

Sentinel Diagnostics — 09/08/2008

F. Rota, L. De Angelis, R. Vannest, B. Nance
Evaluation of the analytical performance of the MULTIGENT Lithium assay on the ABBOTT ARCHITECT c8000 and AEROSET Clinical Chemistry Systems. Verification of the analytical performance according to the HCFA/CLIA directives for allowed Total Error. • Download attachment

Evaluation of MULTIGENT Creatinine (Enzymatic) Liquid on the ABBOTT Clinical Chemistry Systems

Sentinel Diagnostics — 09/08/2008

F. Rota, F.Magro, B. Nance, H. V. Markle
Evaluation of the MULTIGENT Creatinine (Enzymatic) Liquid assay on the ABBOTT ARCHITECT c8000 and AEROSET Clinical Chemistry Systems. Verification of the analytical performance and agreement with the NKDEP recommendations. • Download attachment

Evaluation of SENTINEL CH. Serum Protein Assays on Synchron LX Clinical Chemistry Sistem

Sentinel Diagnostics — 09/08/2008

F. Rota, A. Mezzadra, R. Dioli
Analytical performance of some SENTINEL CH. Protein assays where evaluated on the Beckman Coulter Syncron LX Clinical Chemistry System. Supporting the effort of clinical laboratories to consolidate all the Protein Panel on one unique instrument platform to maximize lab workflow.
(Studies carried out in partnership with Beckman Coulter Inc.) • Download attachment

Automated haemoglobin in faeces collected on cards for screening for colorectal cancer

Sentinel Diagnostics — 31/05/2008

C. G. Fraser, C. M. Matthew, K. McKay, F. A. Carey, R. J. C. Steele
Advantageous combination of a very simple card collection device and faecal immunochemical quantitative test for screening for colorectal cancer. • Download attachment

Evaluation of the new CK-MB Liquid assay on the ABBOTT Clinical Chemistry Systems

Sentinel Diagnostics — 29/05/2008

R. Lucini, F. Magro, F. Rota, M. Fritz, H. Troonen
Evaluation of the CK-MB Liquid assay on the ABBOTT ARCHITECT cSystems and AEROSET Clinical Chemistry Systems. Verification of the analytical performance and agreement with the desirable specifications based on biological variability as Ricos. • Download attachment

New direct Hemoglobin A1c method in full automation

Sentinel Diagnostics — 03/10/2007

M.Tagliazucchi, A.Fortuna, W.Tizzanini, C.Bitella, R.Sansottera, A.Carbonieri
Hemoglobin A1c Direct Latex innovative method for the direct determination of HbA1c on full clinical chemistry automated analyzer with one step procedure without manual blood lysis.

Poster presented at the 39th SIBioC National Congress - Rimini 2nd to 5th October 2007 • Download attachment

Evaluation of RAMP Whole Blood Analyzer for Point of Care - Troponin I and NT-proBNP Testing

Sentinel Diagnostics — 27/06/2007

M.L. Zucker, G.Soffiati, G.Secco, G. Arthur, A. Fortunato

Correlation study of the RAMP testing TnI and NT-proBNP with ADVIA Centaur ( Siemens Medical Diagnostic Solutions ) and with Elecsys ( Roche Diagnostics ).

Poster at the 10th Annual SCPC Congress – Nashville, TN – April, 2007. • Download attachment

CRP Vario – An Immunoassay for Ultrasensitive and Wide-Range Assay of CRP on AEROSET® and ARCHITECT®

Sentinel Diagnostics — 05/07/2005

C-Reactive Protein (CRP) is the best-known among the acute-phase proteins. CRP concentration increase in response to inflammation, postoperative conditions or tissue damage. CRP normally present in low concentration (< 5 mg/L) can raise rapidly during the acute inflammatory process with values up to 500 mg/L during bacterial infections. In recent years, studies have shown that in apparently healthy individuals a direct correlation between the level of CRP and the risk of developing coronary heart disease (CHD) exists.
CRP has also been reported as a potential marker for monitoring Statin therapy. The various clinical applications of CRP require access to both a sensitive and a wide range assay.
CRP Vario was designed to fulfill both requirements. • Download attachment

STUDY OF SAMPLE-TO-SAMPLE AND REAGENT-TO-REAGENT ON AEREOSET® AND ARCHITECT®

Sentinel Diagnostics — 12/05/2005

Modern random access analysers are playing a determining role in the productivity of clinical laboratories and their ability to offer clinicians an ever-wider range of tests with state-ofthe-art analytical performance. Assay sensitivity increasing and the number of on-board reagents expanding, however, the potential for interaction among and between samples, reagents and analyzer has increased, hence, the possibility for a negative impact on patient results. We here report on our brief to verify sample-tosample and reagent-to-reagent carry over for a total of 32 new reagents equating 32 x (31+ 54) x 2 or 5440 reagent combinations on the Abbott AEROSET® with confirmation of carry over on ARCHITECT® c8000 clinical chemisty analyzers. • Download attachment

COMMUTABILITY OF 17 SENTINEL REAGENTS FOR USE ON AEROSET® AND ARCHITECT®

Sentinel Diagnostics — 12/05/2005

Depending on the individual clinicallaboratory needs it is common practice for instrument manufacturers to offer the choice of more than one analyser. These instruments can be entirely different or more often belong to the same family, differing only in terms of capacity and specific performance characteristics.
The goal of this study was to evaluate equivalency of the AEROSET® and ARCHITECT® c8000 (Abbott). Using a validation protocol created for this specific purpose we have compared the performance of 17 IVD products (Sentinel CH, Milan, Italy) on the 2 instruments with regard to precision, accuracy and cross-interference. • Download attachment

EXPANSION OF ABBOTT ANALYZERS REAGENT MENU: VALIDATION OF 17 SENTINEL REAGENTS

Sentinel Diagnostics — 12/05/2005

New tests previously performed using alternative methods (e.g. Atomic Absorption, Nephelometry, Potentiometry) on different instruments, the necessity for clinical laboratories to overhaul and improve their services in terms of turnaround time, breadth and depth of test menu, quality of results, and in general new value added, together with dwindling economic resources and the availability of high-throughput random-access analysers integrating in-line immunochemistry testing modules have significantly changed the nature of laboratory work. Work is no longer strictly partitioned between diagnostic areas and an increasing number of lab activities are being merged. To address this challenge Sentinel and Abbott’s scientific and technical work teams have joined forces. We here report on the outcome of this collaboration for the optimisation of 17 in-vitro diagnostic products (Sentinel) and their validation for routine use of the Abbott AEROSET® and ARCHITECT® c8000 analysers according to the requirements of the European Directive 98/79/CE. • Download attachment

CRP VARIO IMPLEMENTS VARIABLE RANGE MEASUREMENT OF C-REACTIVE PROTEIN ON AEROSET® & ARCHITECT® c8000

Sentinel Diagnostics — 12/05/2005

C-Reactive Protein (CRP) is the bestknown among the acute-phase proteins, the concentration of which increases in response to inflammation, postoperative conditions or tissue damage. CRP is normally present in low concentration (<5 mg/L) but increases rapidly during the acute inflammatory process with values increasing up to 500 mg/L during bacterial infections. The level of CRP can be detected 6 hours after inflammation starts reaching a peak at 48 hours. The determination of CRP represents a useful laboratory test for the detection of acute infection and for monitoring of inflammation in acute rheumatic and gastrointestinal diseases. In recent years, studies have shown that in apparently healthy individuals a direct correlation between the level of CRP and the risk of developing coronary heart disease (CHD) exists. CRP has also been reported as a potential marker for monitoring Statin therapy. • Download attachment