Validation of a New Freeze-Dried PCR Universal Master Mix
AACC 2010, Anaheim - California

16 November 11

M. Gramegna, E. Castiglioni, A. Moiana, M. Zanussi, M. Ferrari
Sentinel Ch. has recently developed a new proprietary freeze-drying system for the manufacturing of ready-to-use PCR Universal Master Mixes. Usually the PCR mixes need to be stored at -20°C or + 2/8 °C. This means request for refrigerators and freezers in the labs, and controlled temperature in case of shipment. The new system is able to maintain the activity of the mix with no loss of performance at room temperature. The aim of the present study is to validate the performances of the freeze-dried mixes for the main molecular applications in research or diagnostic laboratories.